Journal: Aging Cell

Article Title: Novel PMVs / ZIP4 /Zinc/Prelamin A Axis Promotes Nuclear Dysmorphism and Vascular Aging in Humans and Rodents Post‐Injury: Effective Treatment With Platelet Membrane‐Coated ZIF ‐8 Nanoparticles

doi: 10.1111/acel.70443

Figure Lengend Snippet: Demethylation of Lamina‐associated domains (LADs) is associated with nuclear dysmorphism and vascular aging. (A) WGBS detected the DNA methylation in the injured arteries and the self‐control arteries (2 weeks) ( n = 4). Smoothed scatter plots showed the correlation of methylation percentage between the control and the injured arteries throughout the Genome wide CpG, Promoter, Genebody, and Intergenic regions. (B) Schematic representation of the spatial organization of genes within LADs, highlighting the interactions between chromatin and Lamin A. (C) CpG methylation level within the LADs in the injured arteries (purplish red) was significantly repressed compared with that in the control group (black). (D) Integrating RNA‐seq and WGBS data to analyze the correlation between CpG methylation and gene expression levels in LADs. (E) Ingenuity Pathway Analysis (IPA, Qiagen, Venlo, Netherlands) showed that genes within LADs participate in cell senescence. (F) Immunofluorescence staining of Lamin A (red), H3K9me3 (green) and DAPI (blue) in VSMCs after pMVs treatment. Arrows indicate deformed nuclei. (G) Pearson correlation was used to analyze the relationship between nuclear dysmorphism and H3K9me3 expression, and heatmap indicated that H3K9me3 levels were correlated with increased nuclear dysmorphism. Quantification of mean fluorescence intensity of H3K9me3 is shown on the right. The data are shown as mean ± SD, * p < 0.05 (one‐way ANOVA with Bonferroni's multiple comparison post hoc test). n = 3. (I) Representative TEM images showing nuclear ultrastructure in control and pMVs‐treated VSMCs, with quantitative analysis of peri‐laminar heterochromatin thickness. (J) Representative three‐dimensional SIM images of VSMC nuclei stained for Lamin A (red), H3K9me3 (green), and DAPI (blue).

Article Snippet: To further validate these observations in three dimensions, 30‐μm‐thick vascular tissue sections were prepared and subjected to DAPI staining followed by Z‐stack imaging and three‐dimensional reconstruction using Imaris software.

Techniques: DNA Methylation Assay, Control, Methylation, Genome Wide, CpG Methylation Assay, RNA Sequencing, Gene Expression, Immunofluorescence, Staining, Expressing, Fluorescence, Comparison

Journal: Traffic (Copenhagen, Denmark)

Article Title: Clathrins Are Involved in the Endocytosis of Host Cytosol in the Malaria Parasite

doi: 10.1111/tra.70025

Figure Lengend Snippet: Video S1: Dextran‐containing structure in the PfCHC knockdown parasites. A representative three‐dimensional (3D) reconstructed image of a PfCHC knockdown parasite‐infected red blood cell is shown from different angles by this video. A dextran (green)‐containing structure, which is surrendered by the parasite membrane (red, stained by Bodipy‐TR‐C5_ceramide), is localized at parasite periphery. This dextran‐containing structure displays as a long tubular‐like morphology.

Article Snippet: Three‐dimensional (3D) reconstructions and isosurface models in volume images of reconstructed stacks were performed using the IMARIS version 7.7.2 software suite (Bitplane AG).

Techniques:

Journal: Science Progress

Article Title: Association between intracranial artery stenosis and aneurysm rupture risk: Proximal stenosis as a potential protective factor

doi: 10.1177/00368504251346013

Figure Lengend Snippet: Some cases of aneurysms and arterial stenosis (A. ruptured internal carotid artery aneurysm combined with parent artery stenosis; B. unruptured middle cerebral artery aneurysm combined with parent artery stenosis; C. ruptured anterior cerebral artery aneurysm combined with middle cerebral artery stenosis) (red arrow: aneurysm; yellow arrow: arterial stenosis) (CTA-3D: the three-dimensional reconstruction results of computed tomography angiography; CT: the scan result of computed tomography).

Article Snippet: Two neuroradiologists, with 12 and 13 years of experience respectively, independently assessed each aneurysm's origin, determined whether it was a bifurcation aneurysm, checked for the presence of a daughter sac, and evaluated for proximal parent artery stenosis using the reconstructed three-dimensional CTA (GE Discovery CT750 HD or GE Revolution CT or Philips iCT 256) datasets.

Techniques: Computed Tomography